The objective of this research is to develop a methodology that allows the construction of a standard of knowledge management and technological innovation applicable to the field of the universities in Mexico, the use of which results in better use of their resources and the improvement of their productivity. The analysis is carried out based on the systematic integration of the knowledge management and innovation models published in recent years, as well as the results of the experiences that propose standards for knowledge management and innovation. The results found are that knowledge management favors and facilitates innovation in organizations and allows them to offer better products and services. These findings are with the application of a methodology and an instrument to measure the maturity of the organizational structure. The application of the evaluation instrument was applied in a single organization. However, it can be considered a case study that empirically contributes to the state of the art of knowledge management. Organizations need to become aware of the high value of the knowledge and experience in various fields of the people who participate in them. It has been observed that the participation of experts facilitates the achievement of organizational objectives and favors knowledge management and innovation. This study provided information and experiences of an administrative nature to a higher education institution, which allowed an improvement in strategic decision-making, the promotion of technological developments, and the improvement of internal processes that lead to greater effectiveness of the academic and research processes.